RESEARCH ASSISTANT AYŞE EZGİ ÜNLÜ BÜYÜKTOPCU    
Name : AYŞE EZGİ
Surname : ÜNLÜ BÜYÜKTOPCU
E-Mail : aeunlu@eng.ankara.edu.tr
Phone Number : 0-312-2033456
Title : RESEARCH ASSISTANT
Unit : FACULTY OF ENGINEERING
Department : Chemical Engineering
Personal Information

EDUCATION

 

Ph.D, Department of Chemical Engineering, Faculty of Engineering, Ankara University, Ankara,  Turkey, 2012

            Advisor: Prof. Dr. Serpil Takaç

            Thesis: Improvement of Bioprocess Conditions for the Production of Antioxidant by Rhodotorula glutinis Under Different Stress Conditions

 

M.Sc., Department of Chemical Engineering, Faculty of Engineering, Ankara University, Ankara,  Turkey, 2005

            Advisor: Prof. Dr. Serpil Takaç

            Thesis: Effects of Pretreatments on the Enanatioselectivity, Activity and  Stability of Candida rugosa Lipase in the Production of  (S)-Naproxen from Racemic Naproxen Ester

B.S.,   Department of Chemical Engineering, Faculty of Engineering, Ankara University, Ankara, Turkey, 2002

PROFFESSIONAL EXPERIENCE

Research Assistant, Department of Chemical Engineering, Faculty of Engineering, Ankara University, Ankara,  Turkey, 2005- today.

 

GRANTS

Post-Doc Researcher, TUBİTAK 2219, Technical University of Hamburg, Harburg, Germany, January 2014-January 2015.

RESEARCH INTERESTS

1.      Enzymatic Polymerization

2.      Green Solvents

3.      Flavonoids

4.      Enzyme and Protein Productions (Shake-flask and fermentor experiments)

Production of antioxidant enzymes by Rhodotorula glutinis

Production of Candida rugosa lipase

5.      Production of the chiral compound S-Naproxen by Candida rugosa lipase

6.      Proteome analysis of Candida rugosa enyzmes

7.      Experimental Design

8.      Purification and Characterisation of Proteins and Enzymes

Chromatographic separations

PUBLICATIONS

Journal Articles Covered by Science Citation Index:

1.      Ünlü, A. E., Takaç, S. 2017. Improvement of superoxide 
dismutase activity using experimental design and radical 
promoters. Biotechnology and Biotechnological Equipment. 
(accepted)

 

2.      Ünlü, A. E., Takaç, S. 2017. Gliserol varlığında Rhodotorula glutinis çoğalma kinetiğinin incelenmesi ve katalaz aktivitesinin artırılması. Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi. (accepted)

 

3.      Ünlü, A. E., Takaç, S. 2012. Investigation of the simultaneous production of superoxide dismutase and catalase enzymes from Rhodotorula glutinis under different culture conditions. Artificial Cells, Blood Substitutes, and Biotechnology, DOI:10.3109/10731199.2012.668910.

 

4.      Takaç, S., Ünlü, A. E., Erdem, B. 2010. Oxygen Transfer Strategy Modulates the Productions of Lipase and Esterase Enzymes by Candida rugosa, Journal of Molecular Catalysis B: Enzymatic, 64, 150-154. 

 

5.      Takaç, S., Ünlü, A. E. 2009. Effects of alcohol and buffer treatments on the activity and enantioselectivity of Candida rugosa lipase,  Preparative Biochemistry and Biotechnology, 39 (2), 124-141.

 

6.      Takaç, S., Erdem, B., Ünlü, A. E. 2009. Impact of inoculation strategy on the progress of Candida rugosa cultivation. Artificial Celss, Blood Substitutes & Biotechnology 37 (3), 130-137. 

 

Conference Abstracts:

            International

       Ünlü, A. E., Prasad, B., Anavekar, K., Bubenheim, P., Liese, A. 20-21/03/2017. The effect of natural deep eutectic solvent on laccase catalyzed polycatechin synthesis, 2nd International Conference on Enzymology and Molecular Biology (Enzymology & Mol. Biology 2017) p.41, Rome, Italy (oral presentation).

 

2.      Ünlü, A. E., Takaç, S., 20-21/03/2017.  Optimisation of catalase activity by Rhodotorula glutinis using experimental design. 2nd International Conference on Enzymology and Molecular Biology (Enzymology & Mol. Biology 2017) p.90, Rome, Italy.

 

3.      Ünlü, A. E., Anavekar, K., Bubenheim, P., Liese, 31.08-04.09.2014. Enzymatic Polymerization of Catechin in Non-conventional Media, 7th International Congress on Biocatalysis, p.250, Hamburg, Germany.

 

4.      Ünlü, A. E., Erdem, B., Takaç, S. 1-5 /10/2008. Oxygen Transfer Strategy Modulates The Productions Of Lipase And Esterase Enzymes by Candida rugosa, International Enzyme Engineering Symposium (oral presentation).      

  

5.      Ünlü, A. E., Takaç, S., 24.09/01.10/2011 The Effects of pH and Temperature on the Production of Antioxidant Enzymes by Rhodotorula glutinis. European Biotechnology Congress 2011, İstanbul, Current Opinion in Biotechnology, 22S S55, İstanbul, Turkey.

 

6.      Takaç, S., Ünlü, A. E., 21-24/08/2005. Effect of Alcohol Tretament on Hydrolytic Activity of Candida ruogosa Lipase. 12th European Congress on Biotechnology, Journal of Biotechnology 118: S111-S112, Copenhagen, Denmark.

 

7.      Takaç, S., Ünlü, A. E. 21-24/08/2005.  Effect of pH in the 2-propanol Treatment of Candida rugosa Lipase on its Enantioselectivity in the Hydrolysis of Racemic Naproxen Methyl Ester, 12th European Congress on Biotechnology, Journal of Biotechnology 118:S112-S113, Copenhagen, Denmark.

 

8.      Takaç, S., Ünlü, A. E., 29/8-1/9/2004. Enhancement of Activity, Stability, and Enantioselectivity of Candida rugosa Lipase by 2-Propanol Treatment for the Production of S-Naproxen, 2nd International Congress on Biocatalysis (BIOCAT 2004), Book of Abstracts 89, Hamburg, Germany.

 

National


  1. Ünlü, A. E., Değirmenbaşı, D., Takaç, S.,3-6/9/2012. Rhodotorula glutinis’in katalaz aktivitesi üzerine bazı radikal oluşturucuların etkisi, Onuncu Ulusal Kimya Mühendisliği Kongresi (UKMK-10) Bildiri Özetleri Kitabı, İstanbul.

(The effects of the radical sources on the catalase activity of Rhodotorula glutinis)

 

  1. Büyüktopçu, A.E., Erdem, B. Takaç, S., 28-31/10/2007. Candida rugosa Proteinlerinin Klasik Proteombilim Yaklaşımı ile Analizi, 15. Biyoteknoloji Kongresi, Bildiri Özetleri Kitabı, 34, Antalya.

(The analysis of Candida rugosa proteins by proteomics)

 

  1. Erdem, B., Büyüktopçu, A. E., Takaç, S. 5-8/9/2006. İki Fazlı Sistemde Candida rugosa Lipazı ile Rasemik ve S-Naproksen Metil Esterlerin Hidroliz Tepkimelerinin Kinetik Sabitlerinin Bulunması, Yedinci Ulusal Kimya Mühendisliği Kongresi (UKMK-7) Bildiri Özetleri Kitabı, BT023, Eskişehir.

(The investigation of the kinetic parameters of racemic and  S-Naproxen methyl esters hydrolysis reactions at two-phase system by  Candida rugosa lipase)

 

  1. Takaç, S., Ünlü, A. E., Bakkal, M., Mutlu, D., 7-10/9/2004. Lipaz Biyokatalizörlüğünde Enantioseçimli Hidroliz ile S-Naproksen Üretim Tepkimesinin Kinetiği, Altıncı Ulusal Kimya Mühendisliği Kongresi (UKMK-6) Bildiri Özetleri Kitabı, RM11, İzmir. (The kinetics of S-Naproxen production reaction by enantioselective hydrolysisusing lipase)

 

 

 

 PROJECTS

 

1.      Biocatalysed Polymer Synthesis in Non- Conventional Media, Technical University of Hamburg, Harburg, Germany, Reseacher, TUBİTAK, 2014-2015.

2.      Identification of the Microorganisms Isolated for Lipase Production and Purification of the Synthesized Enzymes, Ankara University, BAP, Project no: 2007-07-45-004HPD.

3.      Improvement of Biotechnological Process Conditions by Proteomics for the Production of an Industrial Enzyme, Researcher, TUBITAK, 2005-2007.

4.      Investigation of  Lipase Production Capacities of Microorganisms Isolated from Different Sources and Their Potential Uses in Industry, Ankara University, Institute of Biotechnology, Researcher, 2005-2006.

5.      Investigation of the Process Parameters for the Production of Enantiomerically Pure Drugs, BAP, Proje No: 2004-07-45-025.

6.      Development of a Biotechnological Process for Chiral Pharmaceutical S-Naproxen, Ankara  University, Institute of Biotechnology, Researcher, 2003-2005.