DR.ÖĞR.ÜYESİ ARZU ZEYNEP KARABAY    
Name : ARZU ZEYNEP
Surname : KARABAY
E-Mail : akarabay@ankara.edu.tr, zeynepkarabay@yahoo.com
Phone Number : 203 30 60
Title : DR.ÖĞR.ÜYESİ
Unit : FACULTY OF PHARMACY
Department : BIOCHEMISTRY
Personal Information

 

CURRICULUM VITAE


 

PERSONAL INFORMATION                                                       

Name-surname: Arzu Z. Karabay  

 

EDUCATION 

·         2005-2011: Ph.D. in Biochemistry. Ankara University Faculty of Pharmacy, Department of Biochemistry, Turkey.

Supervised by Prof. Zeliha Buyukbingol.

·         2002-2005: M. Sc. in Biochemistry. Ankara University Faculty of Pharmacy, Department of Biochemistry, Turkey.

Supervised by Prof. Zeliha Buyukbingol.

·         1997-2002: B.Sc. in Pharmacy. Ankara University Faculty of Pharmacy, Turkey.

·         1996-1999: Konya Meram Anatolian High School, Turkey.

M.Sc. THESIS TOPIC

·         Probable antioxidant effects of tryptophan on streptozotocin treated diabetic rats.

(Supervised by Prof. Zeliha Buyukbingol).

Ph.D. THESIS TOPIC

·         Effects of methylsulfonylmethane, glucosamine and chondroitin sulfate on apoptosis in LPS/IFN-γ activated RAW 264. 7 macrophage cells. (Supervised by Prof. Zeliha Buyukbingol).

RESEARCH INTERESTS

·         Apoptosis, autophagy, NF-kB, cell signaling in cancer, nitric oxide, chronic inflammation, drug resistance in cancer

WORK EXPERIENCE

 2014-2017: Assistant Professor (Ankara University Faculty of Pharmacy, Department of Biochemistry).

2003-2014: Research assistant (Ankara University Faculty of Pharmacy, Department of Biochemistry).

·     1 May-31 July 2008: Internship in the laboratory of Prof. Beatriz de las Heras Polo, Complutense University of Madrid, Faculty of Pharmacy, Department of Pharmacology, Madrid, Spain (Scholarship from Erasmus Mobility Programme).

              

PUBLICATIONS

 

A-PUBLICATIONS (JOURNALS INDEXED IN SCI)

A1. Expression analysis of Akirin-2, NFκB-p65 and β-catenin proteins in imatinib resistance of chronic myeloid leukemia. Karabay AZ, Koc A, Ozkan T, Hekmatshoar Y, Altinok Gunes B, Sunguroglu A, Buyukbingol Z, Atalay A, Aktan F. Hematology. 2018 Jun 26:1-6. doi: 10.1080/10245332.2018.1488795

A2. Characterization of imatinib-resistant K562 cell line displaying resistance mechanisms Yalda Hekmatshoar, Tulin Ozkan, Buket Altinok Gunes, Sureyya Bozkurt, Aynur Karadag, Arzu Zeynep  Karabay, Asuman Sunguroglu Doi: http://dx.doi.org/10.14715/cmb/2018.64.6.5

A3. Food Additive Sodium Benzoate (NaB) Activates NFκB and Induces Apoptosis in HCT116 Cells. Yilmaz B, Karabay AZ. Molecules. 2018 Mar 22;23(4). pii: E723. doi: 0.3390/molecules23040723.

A4. Apoptotic effects of some tetrahydronaphthalene derivatives on K562 human chronic myelogenous leukemia cell line. Koç A, Ozkan T, Karabay AZ, Hekmatshoar Y, Gurkan-Alp AS, Aktan F, Buyukbingol Z, Sunguroglu A, Buyukbingol E. Anticancer Agents Med Chem. 2018 Feb 7;17(14):1924-1930. doi: 10.2174/1871521409666170412122811. 

A5. Intracavernous Injection of Human Umbilical Cord Blood Mononuclear Cells Improves Erectile Dysfunction in Streptozotocin-Induced Diabetic Rats. Cengiz T, Kaya E, Oral DY, Ozakca I, Bayatli N, Karabay AZ, Ensari TA, Karahan T, Yilmaz E, Gur S. J Sex Med. 2017 Jan;14(1):50-58. 

A6. Methylsulfonylmethane Induces p53 Independent Apoptosis in HCT-116 Colon Cancer Cells. Karabay AZ, Koc A, Ozkan T, Hekmatshoar Y, Sunguroglu A, Aktan F, Buyukbingol Z. Int J Mol Sci. 2016 Jul 15;17(7).

A7. Inhibitory effects of indole α-lipoic acid derivatives on nitric oxide production in LPS/IFNγ activated RAW 264.7 macrophages. Karabay AZ, Koc A, Gurkan S, Buyukbingol Z, Buyukbingol E. Cell Biochem Funct. 2015 Apr;33(3):121-7. 

 

A8. Methylsulfonylmethane modulates apoptosis of LPS/IFN-γ-activated RAW 264.7 macrophage-like cells by targeting p53, Bax, Bcl-2, cytochrome c and PARP proteins.

Karabay AZ, Aktan F, Sunguroğlu A, Buyukbingol Z. Immunopharmacol Immunotoxicol. 2014 Sep (11):1-11.

 

A9. Effect of L-carnitine on the synthesis of nitric oxide in RAW 264·7 murine macrophage cell line. Koc A, Ozkan T, Karabay AZ, Sunguroglu A, Aktan F. Cell Biochem Funct. 2011 Dec;29(8):679-85. 

 

A10. Synthesis and effects of some novel tetrahydronaphthalene derivatives on proliferation and nitric oxide production in lipopolysaccharide activated Raw 264.7 macrophages.

Gurkan AS, Karabay AZ, Buyukbingol Z, Buyukbingol E. Eur J Med Chem. 2011 Feb;46(2):468-79. 

 

A11. Syntheses of novel indole lipoic acid derivatives and their antioxidant effects on lipid peroxidation. Gurkan AS, Karabay A, Buyukbingol Z, Adejare A, Buyukbingol E. Arch Pharm (Weinheim). 2005 Mar;338(2-3):67-73.

 

 

PUBLICATIONS (PEER REVIEWED JOURNALS IN OTHER INDEXES)

 

A12. İmatinib’e Duyarlı ve Dirençli K562 Hücrelerinde Kalneksinin Protein Ekspresyonunun Araștırılması Arzu Z. Karabay Ankara Üniversitesi Tıp Fakültesi Mecmuası 2018, 71 (1) DOI: 10.1501/Tıpfak_000000990

 

A13. L-Karnitin ve alfa-lipoik asitin, HCT 116 +/+ ve -/- kolon kanser hücre serilerinin proliferasyonu üzerindeki sinerjetik etkileri Yazar(lar):KOÇ, Aslı; KARABAY, Arzu Z; Özkan, Tülin; Büyükbingöl, Zeliha; Sunguroğlu, Asuman; Aktan, Fügen Cilt:15 Sayı:2 Sayfa: 001- 08 DOI:10.1501/Ashd_0000000114 Yayın Tarihi:2016

 

A14. Akut miyeloid lösemide akirin2 geninin ifadesi Yazar(lar):Özkan, Tülin; Altınok, Buket; Karadağ, Aynur; Karabay, Arzu Z; Hekmatshoar, Yalda; Sayınalp, Nilgün; Sunguroğlu, Asuman Cilt:15 Sayı:1Sayfa:019-023 DOI:10.1501/Ashd_0000000112 Yayın Tarihi:2016

A15. Effects of glucosamine on LPS/IFN-γ induced RAW 264.7 macrophage apoptosis. A. Z. Karabay, A. Koc , T. Ozkan, A. Sunguroglu, F. Aktan, Z. Buyukbingol. Ankara Üniversitesi Tıp Fakültesi Mecmuası 2012, 65(1) 11-18, 2012.

A16. Effect of taurine on hydrogen peroxide production in lipopolysaccharide induced RAW 264.7 macrophage cells. Arzu Z. Karabay, Aslı Koç, Tülin Özkan, Zeliha Büyükbingöl, Asuman Sunguroğlu, Fügen Aktan. FABAD J. Pharm. Sci., 33, 187-192, 2008.

 

 

B-POSTERS AND ORAL PRESENTATIONS

 

 

B1. Alper Yılmaz, Arzu Z. Karabay CoQ0 (Coenzyme Q0) decreases nitrite levels in IFN-γ activated RAW 264.7 macrophages 7th International Molecular Biology and Biotechnology Congress Konya, Turkey 25-27 Nisan 2018 (Sözlü Sunum)

 

B2. Arzu Z. Karabay Nilotinib Modulates Inflammatory Pathways İn Macrophages Through İts Effects On COX-2 And Mapk Pathways. Uluslararası Katılımlı 6. İlaç Kimyası Kongresi 22-25 Mart 2018 Antalya (Sözlü Sunum)

 

B3. Arzu Z. Karabay, Asli Koc, Yalda Hekmatshoar, Aynur Karadag, Tulin Ozkan Aspirin Inhibits Bcr-Abl and Its Downstream Effectors in Chronic Myeloid Leukemia. VI. International Congress of Molecular Medicine 22-25 May 2017 Istanbul University Congress and Cultural Center, İstanbul, Turkey (Oral Presentation)

 

B4. Effects of indole α-lipoic acıd derivatıves on cell viability parameters in k562 leukemia cell line Arzu Z. Karabay, Asli Koc, Selen Gurkan, Zeliha Buyukbingol, Erdem Buyukbingol 11th International Symposium on Pharmaceutical Sciences June 09-12, 2015, Ankara Turkey (Oral Presentation)

 

B5. A. Z. Karabay , A. Koc, F. Aktan , Z. Buyukbingol Effect of microenvironment on Imatinib resistance of K562 cells 40th FEBS Congress (FEBS 2015), Berlin 4–9 Jul 2015

 

B6. A. Koc, A. Z. Karabay, T. Ozkan, B. Altinok, Z. Buyukbingol, A. Sunguroglu, F. Aktan. Differences between akirin protein levels in imatinib resistant and sensitive K562 cells. Molecular Life Sciences. 3-6 October 2013, Frankfurt-Germany.


B7. Arzu Z. Karabay, Asli Koc, Selen Gurkan, Zeliha Buyukbingol, Erdem Buyukbingol.Effects of indole α-lipoic acid derivatives on nitric oxide production in LPS/IFN-γ activated RAW 264.7 macrophages FEBS-EMBO Congress, 30 August-4 September 2014, Paris, France

 

B8. Arzu Zeynep Karabay, Tulin Ozkan, Asli Koç, Asuman Sunguroglu, Zeliha Buyukbingol. Effects of methylsulfonylmethane on viability of hct-116 cell line
22th IUBMB/37th FEBS Congress, 4-9 September 2012, Sevilla, SPAIN.

 

B9. Zeliha Buyukbingol, Arzu Zeynep Karabay, Tulin Ozkan, Asuman Sunguroglu.  Effect of methylsulfonylmethane on inos gene methylation in macrophages
22th IUBMB/37th FEBS Congress, 4-9 September 2012, Sevilla, SPAIN.

 

B10.  Ozkan T, Karadag A, Altinok B, Karabay AZ, Koc A, Bozkurt S, Aydos S, Sunguroglu A. NF-kappaB activation and complex control of NOS2 gene promoter in K562 imatinib mesylate resistant CML cell line. 35th Congress of the FEBS, JUN 26-JUL 01,2010, Gothenburg, Sweden.

 

B11. Karabay AZ, Ozkan T, Koc A, Sunguroglu A, Buyukbingol Z. Effect of methylsulfonylmethane on LPS-IFN-gamma induced RAW 264.7 macrophage apoptosis. 35th Congress of the FEBS, JUN 26-JUL 01,2010, Gothenburg, Sweden.

 

B12. Ozkan T, Karadag A, Altinok B, Karabay AZ, Koc A, Bozkurt S, Aydos S, Sunguroglu A. Effect of wnt3a or lithium chloride preconditioning on nitric oxide production in RAW264.7 macrophages and K562 leukemic cell line. 35th Congress of the FEBS, JUN26-JUL01,2010, Gothenburg, Sweden.

 

B13. Asli Akinci, Arzu Karabay, Tulin Özkan, Asuman Sunguroglu, Fugen Aktan. The effect of LPS incubation periods on nitric oxide levels in RAW 264.7 macrophage cell line. 34th FEBS Congress, July 4 - 9, 2009, Prague, Czech Republic.

 


 


 

TEACHING EXPERIENCES

 

Cancer Biochemistry (Post-graduate)

Cell Culture Theory and Practice (Post-graduate)

Tissue Biochemistry (Post-graduate)

Seperation of biological organelles (Post-graduate)

Biochemistry (Undergraduate), Biochemistry Practice (Undergraduate)

Clinical Biochemistry (Undergraduate)  

 

PROJECTS

 

Project Title: Effects of methylsulfonylmethane, glucosamine and chondroitine sulfate on apoptosis and iNOS gene methylation in LPS/IFN-gamma induced RAW 264.7 macrophage cell line. Supported by: The Scientific and Technological Research Council of Turkey. Project number: 110S240 Duty in the Project: Researcher. Project start and finish date: 15.10.2010-15.10.2011

 

Project Title: Interaction of beta-catenin, NF kappaB and akirin proteins and their roles in imatinib resistant chronic myeloid leukemia. Supported by: The Scientific and Technological Research Council of Turkey. Project number: 111T358 Duty in the Project: Researcher. Project start and finish date: 15.10.2011-15.10.2013

 

Project Title: The role of exosome derived miRNA transfer in the acquisition of Imatinib resistance in K562 cells. Supported by: The Scientific and Technological Research Council of Turkey. Project number: 110Z240 Duty in the Project: Supervisor. Project start and finish date: 15.10.2015-Continues

 

Project Title: Apoptotic effects of Probucol on K562 Chronic Myelogenous Leukemia Cell line. Supported by: Ankara University Scientific Research Project Project number: 16H0237014 Duty in the Project: Supervisor. Project start and finish date: 09.11.2016 - 09.05.2017

 

Project Title: Effects of sodium benzoate on viability of HCT116 colon cancer cells. Supported by: Ankara University Scientific Research Project Project number: 16L0237007 Duty in the Project: Supervisor. Project start and finish date: 09.11.2016 - 09.05.2017

 

 

 

MEMBERSHIPS

·         Turkish Biochemical Society

 

LABORATORY SKILLS

 

  • DNA isolation (Genomic DNA, Plasmid)
  • RNA isolation (Tissue, Cell),
  • Electrophoresis:
  • Agarose
  • PAGE, SDS-PAGE
  • Agilent 2100 Bioanalyser
  • PCR and RFLP
  • MSPCR
  • cDNA synthesis
  • QRT-PCR
  • Western blot
  • Microarray (Affymetrix) (Expression-array, Promotor array,)
  • ChIP-on-chip (chromatin immunoprecipitation, Methyl-DNA immunoprecipitation _MEDIP)
  • Primer designing for sequencing, RT-PCR

 

Cell Culture

 

  • Primary cell culture
  • Cell Line Culture (cardiomyocytes, Chronic myeloid leukemia, Acut myeloid leukemia, Sertoli cells, Glioblastoma cells, macrophages, cancer cell lines)
  • Stem Cell Isolation and culture (mesenchymal stem cell, Hematopoetic stem cell(CD34+), cardiac stem cell, Amniotic stem cell (CD117+)
  • Transfection (Plasmid)
  • Cell Proliferation Analysis
  • Immunocytochemistry (IF/IHC)
  • Flow Cytometry

 

Language

 

·         English (Fluent)

·         Turkish (Mother Tongue)

·         German, Spanish (Basic)

HOBBIES

·         Theater,  music,  reading,  travelling,……


 

REFERENCES

 

Prof. Zeliha Buyukbingol

Ankara University Faculty of Pharmacy

Department of Biochemistry

06100, Tandogan, Ankara TURKEY

e-mail: zbuyukbingol@ankara.edu.tr

00 90 312 203 30 53 (Work)

00 90 533 322 63 05 (Mobile)

 

 

 

Prof. Beatriz de las Heras Polo

Faculty of Pharmacy, Deparment of Pharmacology

Plaza de Ramón y Cajal

Ciudad Universitaria

28040 - Madrid, SPAIN

e-mail:lasheras@farm.ucm.es

Tel: 913941873

Fax:913941726